Epigenetics is the study of heritable changes in gene function that occur without a change in the sequence of the DNA, or a change in phenotype without a change in genotype. Epigenetic changes are regular and natural occurrences but can also be influenced by several factors including age, the environment/lifestyle, and disease state.

Epigenetics is known to play a crucial role in development. There are thousands of genes in a mammalian genome. However these genes are expressed or silenced differently in hundreds of cell types (e.g. neuron, heart, kidney, skin) throughout the body. Therefore epigenetics is the crucial factor in cell differentiation and development.

Secondly gene expression in mammalian cells changes in response to external factors, such as viral infection, stress, drug delivery and other events. Epigenetics makes up the link between the environment and gene expression.

At least three systems including DNA methylation, histone modification and non-coding RNA-associated gene silencing are currently considered to initiate and sustain epigenetic change. New and ongoing research is continuously uncovering the role of epigenetics in a variety of human and mammalian disorders and fatal diseases, but also in plant research.

NXT-Dx offers services to study all three epigenetic systems in your samples.

 

For DNA methylation:

- Infinium Methylation EPIC array

- MBD-sequencing or MeDIP-seq

- Reduced Representation Bisulfite Sequencing (RRBS)

- (Oxidative) Bisulfite sequencing (targeted, genome-wide, whole genome)

 

For histone modifications:

- ChIP-sequencing

 

For ncRNAs and miRNAs:

- RNA-seq

- Expression arrays

- Targeted qPCR

 

For more information, send us an email on maarten.braspenning{at}nxt-dx.com and receive a reply within 24hrs.